EXPLORING THE ANTIDIABETIC AND ANTIOXIDANT POTENTIAL OF NEPETA ADENOPHYTA HEDGE IN HEPG2 CELLS AND ITS PHYTOCHEMICAL STUDIES

The current study is based on the phytochemical isolation and biological activity evaluation of Nepeta adenophyta Hedge. This plant species is traditionally used for abdominal pain, kidney pain, stomach problems, urine problems, and diarrhea and is examined to control bleeding. Five compounds were isolated using column chromatography over normal silica gel from the ethanolic crude extract of Nepeta adenophyta and their structures were characterized with the help of spectroscopic techniques. In addition, the crude extract was evaluated for its antioxidant and antidiabetic potential in a human hepatoma cell line HepG2 cells and the crude extract showed good activities. The phytochemical isolation studies and the biological activities are conducted for the first time on the study sample


INTRODUCTION
weakness of their cattle [1]. In a recent study, N.

Solvents and reagents
The solvents of commercial grade n-

Plant Material
The plant material of N. adenophyta Hedge was collected from Astore, Gilgit-

Isolation & Characterization
The crude extract NAE (460 g) was subjected to

Antidiabetic Activity
The antidiabetic activity was performed following the reported protocol [16].

Antioxidant Activity
Hydrogen peroxide-induced oxidative stress was determined in HepG2 cells to check
The structures of 1-5 were elucidated by various spectral techniques. Moreover, the spectral data were compared and found in agreement with the literature.   phthalate. The experimental data was found in correlation with previous literature [19]. 1  The experimental data was found in correlation with previous literature [22]. 1

Results of Antidiabetic Activity
Based on the consumption of glucose assay on HepG2 cells, antidiabetic activity of NAE was carried out. The results are shown in the Table 1.   Table   1. The glucose uptake in HepG2 cells increased with increasing concentrations of sample, i.e., 25, 50, and 100 μg/mL as shown in Fig. 3. The ethanol extract was found active. Significant activity (p<0.05) was found at all tested concentrations.

Results of Antioxidant Activity
The antioxidant potential of the test sample was determined in HepG2 cells. For almost six hours, the cells were treated with 200 μM hydrogen peroxide, and the results are shown in Table 2, which also shows the results for the control group, the model group and quercetin.   which are due to the presence of phenolic and flavonoid content [25]. N. hindostana, N. graciliflora and N. cataria have previously shown high potential for antioxidant, antiinflammatory and anti-diabetic properties [26].
Similarly, recently, N. baytopii, Turkey's endemic specie, has been reported for its antioxidant and antidiabetic potential [27] and N.
deflersiana has exhibited high content of antibacterial and antidiabetic properties [28].
Herein, Nepeta adenophyta Hedge showed antioxidant and antidiabetic potential, which favors its traditional uses and could be an important source in search of medicine for such diseases in future.

CONCLUSIONS
The Himalayan Nepeta adenophyta Hedge was studied for the isolation, and five